Search results for: 'LIGHT'

HVEM, LIGHT & BTLA: New Immunotherapy Target The checkpoint receptors  HVEM ,  LIGHT ,  CD160 , and  BTLA  are part of a complex network of overlapping receptor interactions that function in both immune stimulation and suppression 1 ,  2  .  This regulatory function has made them therapeutic targets for treatment of cancer, autoimmune diseases and allergies, and for improved methods of organ transplants.  While presenting opportunities for...

Fluorescence Polarization Assays: Principles & Applications Fluorescence Polarization (FP) technology measures changes in light polarization emitted by a fluorescent tracer in a sample and is quite different from fluorescence intensity, which measures the intensity of emitted light at a specific wavelength. FP is widely used to monitor molecular interactions in solution as it is easily amenable to high-throughput formats and screening applications. FP is a complex technique that requires car...

PARP/PARPTrap™ Screening & Profiling PARP (Poly ADP-Ribose Polymerase) proteins are a large family of 17 members that catalyze the ADP-ribosylation of proteins and DNA. PARPs are part of a network of 150 proteins involved in the DNA damage response, which constantly scan and repair DNA to maintain genome integrity. The PARP proteins are involved in a wide range of biological functions: DNA repair, chromatin remodeling, mitotic spindle assembly, regulati...

PARP Assays Poly(ADP-ribose) polymerase (PARP) proteins play a crucial role in DNA repair mechanisms within cells. These enzymes are involved in the repair of single-strand DNA breaks through the base excision repair pathway. Inhibition of PARP proteins has emerged as a promising strategy in cancer therapy, particularly in tumors with defective DNA repair mechanisms, such as those with BRCA mutations. When PARP is inhibited, it prevents the repair of single-strand breaks, leading to th...

TR-FRET Assays Simplify and Accelerate Drug Discovery Introduction Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) is a powerful technique commonly used to analyze the binding of two interacting molecules. Since most biological responses involve an interaction between at least two partners, TR-FRET is well suited to study a wide range of events, including many that characterize cellular signaling pathways. Possible applications are limitless: interaction ...

Assay Kit Frequently Asked Questions FAQ Topic: -Any- General Technical Questions IC50 Questions Fluorescence Polarization AlphaLISA® TR-FRET Luminescence FAQ Search: Do ...

Cell Lines and Primary Cells Cell lines are extremely useful tools for the study of protein interactions, gene activation, cell signaling, viral infection, cell-to-cell communication, and other biological processes in the context of an intact cell system rather than cell-free biochemical assays. BPS Bioscience has developed over 250 stable cell lines applicable across a wide range of research areas including immunotherapy, CAR-T cell therapy, cell signaling, metabolism, neuroscience, and mu...

Technical Notes and Application Notes Immunotherapy | Cancer Targets | CAR-T/NK Cell Therapy | Gene & Cell Therapy | Cell Culture | Cancer Pathways | CRISPR/Cas9 | Cell Receptor Targets | Ubiquitination | Protein Conjugation | Technology | Cholesterol Pathways | SARS-CoV-2/COVID-19 Immunotherapy A2aR & Immunotherapy BCMA & Cancer Bispecific Antibodies Combination Therapies for Targetin...

SARS-CoV-2 Antibody Detection SARS-CoV-2 has spread across the globe effectively at a rapid pace causing a worldwide pandemic. Infection with this highly contagious respiratory virus can be asymptomatic or present as COVID-19, a disease with varying degrees of severity. Clinical symptoms may include fever, dry cough, anosmia, gastrointestinal symptoms, hypercoagulability, hyperimmune response (cytokine storm), and acute respiratory distress syndrome (ARDS) with disease progression (1). Patie...

Protein Binding Studies / BLI Detection Services Bio-Layer Interferometry (BLI) is a powerful analytical technique for studying biomolecular interactions. BLI measures changes in interference patterns of light reflected from a biosensor tip, which is typically coated with a layer of molecules relevant to the study. As biomolecules bind to or dissociate from the biosensor surface, the interference patterns change, providing real-time data on the kinetics and affinity of these int...

Frequently Asked Questions BPS Bioscience is here to answer your questions and provide project guidance. On the following pages, we outline some of the most frequently asked questions separated by product type. If your question is not answered through these resources, please contact us today and we are happy to assist.  Proteins Cell Lines Assay Kits ...

Setting a Trap for PARP1 and PARP2 Summary PARP1 and PARP2 operate in DNA damage response (DDR) by sensing single breaks in DNA and by recruiting the repair machinery through poly-ADP ribosylation of histones and other proteins. PARP inhibitors are cytotoxic to cancer cells deficient in repair pathways that are complementary to PARP pathways, and it has been shown that some of these inhibitors act by trapping PARP1/2 onto the DNA, preventing repair and leading to cell death. Thus, DNA tra...

Mechanism of JAK-STAT Signaling in Cancer Cell Signaling Pathways Understanding cell to cell communication through cytokines and other secreted factors is at the center of daily life for countless pharmaceutical researchers.  This is because cytokine-based communication is central to normal development and is often a dysfunctional step in cancer and autoimmune diseases ( 1, 2 ).  It is also of increasing importance because cytokine release syndrome is a leading caus...

IgG Antibodies and Fcγ Receptors in Immunotherapy IgG Antibodies and Fcγ Receptors in Immunotherapy Monoclonal, immunoglobulin G (IgG), antibodies are the basis of powerful therapies for treating cancer, viral infections, and autoimmune disease.  The power of therapeutic antibodies comes from their ability to bind antigen targets with high specificity and then harness the functions of the larger the immune system.  There are a variety of immune responses. Cytotoxic anti...

Cell Line Frequently Asked Questions FAQ Search: Custom Cell Line Development Generate stable cell lines for protein overexpression, mutations or gene knock-out. The development process involves pre-defined milestones with data provided at the completion of each milestone. Each project is customized. Learn more How should I store the cells? Cells are shipped in dry ice, and upon receipt should immediately be thawed for culture...

Research Applications for Engineered iPS Cells Introduction New avenues of research were dramatically opened by the discovery of Takahashi and Yamanaka in 2006 that the ectopic expression of Oct4 (octamer-binding transcription factor 4), Sox2 (SRY-box2), Kfl4 (Kruppel-like factor 4) and c-Myc was sufficient to reprogram terminally differentiated cells into induced pluripotent stem (iPS) cells. These have infinite expansion potential and similar genetic markers, epigenetics and multilineag...

Citations   This page contains a list of scientific journal articles that have cited BPS products and services since 2013. We would like to congratulate all our loyal customers on their contributions to the scientific community. Our customers are truly amazing and dedicated scientists and this is evidenced by the outstanding research they are doing.  Search By Product Name or Catalog Number Name Title 10X HAT Assay Buffer (50095...

Immunotherapy Biochemical Screening Services Immunotherapy is a type of medical treatment that engages the body's immune system to fight diseases, such as cancer, infections, and autoimmune disorders. It works by either stimulating the immune system to attack cancer cells or other disease-causing agents, or by using synthetic proteins or antibodies to target specific molecules on the surface of cancer cells or other pathogens. Immunotherapy has shown prom...