ZAK, GST-tag (Variant 2) Recombinant

Catalog #

40297

$410 *

Size: 10 µg

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Description

Variant 2 recombinant human ZAK, full length, Genbank Accession Number NM_133646.2, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.

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Synonyms

AZK, MLT, MRK, MLK7, MLTK, mlklak

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥75%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

82 kDa

Amino Acids

full length

Genbank #

NM_133646.2

UniProt #

Q9NYL2

Tag(s)

N-terminal GST-tag

Background

ZAK is a member of the MAPKKK family of signal transduction molecules and mediates gamma radiation signaling leading to cell cycle arrest. The activity of ZAK plays a role in cell cycle checkpoint regulation as well as being involved in regulating actin organization (1). Expression of kinase-dead ZAK in mouse fibroblasts disrupts actin stress fibers and causes morphologic changes. ZAK can activate JNK through MKK4/MKK7 and ERK5/p38-gamma via MKK3/MKK6. Expression of ZAK increases the population of cells in the G2/M phase of the cell cycle, whereas dominant-negative ZAK attenuated the G2 arrest caused by gamma radiation (2).

References

1. Yang J-J, et al: Mixed lineage kinase ZAK utilizing MKK7 and not MKK4 to activate the c-Jun N-terminal kinase and playing a role in the cell arrest. Biochem. Biophys. Res. Commun. 297: 105-110, 2002.
2. Gross E. A, et al: MRK, a mixed lineage kinase-related molecule that plays a role in gamma-radiation-induced cell cycle arrest. J. Biol. Chem. 277: 13873-13882, 2002

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ZAK, GST-tag (Variant 2) Recombinant

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