MLK3, GST-tag Recombinant

Catalog #

41104

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human MLK3, also known as Mixed Lineage Kinase 3, Mitogen-activated protein kinase kinase kinase 11, MAP3K11, PTK1, SPRK, and MGC17114, GenBank Accession No. NM_002419, a.a. 1-488, with N-terminal GST-tag, MW = 83 kDa, expressed in Sf9 cells via a baculovirus expression system.

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Synonyms

Mixed Lineage Kinase 3, Mitogen-activated protein kinase kinase kinase 11, MAP3K11, PTK1, SPRK, MGC17114

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 Insect Cells

Purity

≥75%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

83 kDa

Amino Acids

1-488

Specific Activity

9.5 pmole/min/μg

Genbank #

NM_002419

UniProt #

Q16584

Tag(s)

N-terminal GST-tag

References

1. Ing, Y.L. et al., Oncogene. 1994. 9:1745-1750.
2. Chadee, D.N. et al., Nature Cell Biol. 2004. 6:770-776.

Storage/Stability

>6 months at –80°C.

Assay Conditions

Varying concentrations of MLK3 were mixed with 5 μg of MBP protein substrate and 5 μl of 250 μM [³³P]-ATP was added for a total volume of 25 μl. Mixture was incubated for 15 min at 30°C. Reaction was stopped by placing 20 μl of reaction onto phosphocellulose P81 paper. Paper was washed 3 times with 1% phosphoric acid solution for 10 min each and air-dried. Radioactivity was measured from the P81 paper in the presence of scintillation fluid using a scintillation counter.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Serine-Threonine Kinase

Regulatory Status

Research Use Only

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41104_Lotxxxxxx.pdf

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