Tie2, His-tag Recombinant

Catalog #

40270

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human Tie2 (GenBank Accession No. NM_000459), a.a. 771-end with N-terminal His-tag, MW=41 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

TIE, TEK, Tyrosine Kinase With Immunoglobulin-Like And EGF-Like Domains 1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥74%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

41 kDa

Amino Acids

771–end

Specific Activity

140 U/µg

Unit Definition

One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substarte per minute at pH 7.4 and 30°C.

Genbank #

NM_000459

UniProt #

Q02763

Tag(s)

N-terminal His-tag

References

1. Ramsden, J.D., et al., Endocrinology 146 (7), 2895-2902 (2005).
2. Tammela,T., et al., Blood 105 (12), 4642-4648 (2005).

Storage/Stability

>6 months at –80°C.

Assay Conditions

50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP, 30 µg/ml Poly (Glu:Tyr)4:1 substrate, and 2 µg/ml recombinant Tie2.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40270_Lot130205-3C.pdf

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