Sortase A Pentamutant, S. aureus, His-tag Recombinant
Catalog #
71046
$535
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Description
Recombinant Staphylococcus aureus Sortase A (srtA), encompassing amino acids 26-206(end). This construct contains an N-terminal His-tag (6xHis). The protein contains five mutations of interest (P94R), (D160N), (D165A), (K190E), and (K196T) and it is calcium dependent. The recombinant protein was affinity purified.
This product has been cited 2 times.
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Synonyms
Surface protein sorting A, srtA
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Product Data Gallery
4-20% SDS-PAGE Coomassie Staining
Sortase A Activity
Product Info
Storage and Usage
Citations2
Species
S. aureus
Construct
Sortase A (His-26-206(end)) (S. aureus)
Host Species/Expression System
E. coli
Purity
≥90%
Format
Aqueous buffer solution
Formulation
25 mM MES, pH 6.5, 382 mM NaCl, and 10% Glycerol
MW
22 kDa
Amino Acids
26-206(end)
Genbank #
AF162687
UniProt #
Q2FV99
Background
Staphylococcal Sortase A is a bacterial transpeptidase that covalently attaches proteins to the bacterial cell wall by cleaving between threonine and glycine at an LPXTG recognition motif and catalyzes the formation of an amide bond between the carboxyl-group of threonine and the amino-group of the cell-wall peptidoglycan. This chemistry has been exploited as a molecular "stapler" to site-specifically link proteins with the C-terminal LPETGX motif to other proteins or molecules possessing a glycine or aminomethylene motif. The Sortase A Pentamutant exhibits higher activity than the wild type protein. This reaction is applicable to proteins in solution and on the living cell surface.
References
1. Chen, I. et al., Proc Natl. Acad. Sci. (USA). 2011; 108(28): 11399-11404.
2. Kappel, K., et al., Protein Sci. 2012 Dec;21(12):1858-71.
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