Ca2+ Independent Sortase, His-Tag (S. aureus) Recombinant

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Catalog #

100666

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Description

Recombinant Staphylococcus aureus Sortase A, encompassing amino acids 26-206 (end). The construct contains an N-terminal His-tag (6xHis). The protein contains seven mutations (P94R, E105K, E108A, D160N, D165A, K190E, K196T) and is Ca+ Independent. The recombinant protein was affinity purified.

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Synonyms

Class A sortase SrtA, A Staphylococcus aureus transpeptidase, sortase A

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

Product Info

Storage and Usage

Citations

Species

S. aureus

Mutation

P94R, E105K, E108A, D160N, D165A, K190E, K196T

Host Species/Expression System

E. coli

Purity

≥90%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 400 mM imidazole

22 kDa

Amino Acids

26-206(end)

Genbank #

AF162687

UniProt #

Q2FV99

Tag(s)

N-terminal His-tag

References

Hirakawa H., et al., 2015 Biotechnol J 10(9): 1487-92.

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Datasheets/Protocols

100666 Lot 200831-A

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Ca2+ Independent Sortase, His-Tag (S. aureus) Recombinant

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