PDK1, GST-tag

Catalog #: 40080
Size: 10 µg
$345
Species: Human
Host Species: Sf9 insect cells
MW: 89 kDa
Genbank #: NM_002613
Tag(s): N-terminal GST-tag
a.a: full length
Background:
PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns(3,4,5)P3 or PtdIns(3,4)P2. PDK1 then activates protein kinase B (PKB) which, in turn, inactivates glycogen synthase kinase-3 (GSK3). The phosphorylation of other proteins by PKB and GSK3 is likely to mediate many of the intracellular actions of insulin. Thus, PDK1 plays a key role in mediating many of the actions of the second messenger(s) PtdIns(3,4, 5)P3 and/or PtdIns(3,4)P2. The human PDK1 is a 556-residue monomeric enzyme comprising of a catalytic domain that is most similar to the PKA, PKB and PKC subfamily of protein kinases.
Description:
Human 3-phosphoinositide-dependent protein kinase (PDK1), GenBank Accession No. NM_002613, full length with N-terminal GST-tag, MW=89 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Synonym(s):
PRO0461; PDPK1; MGC20087; MGC35290; PkB-like; PkB-like 1 ,
Specific Activity:
≥100 pmol/min/µg
Assay Conditions:
Assay was done in Kinase buffer containing 0.2 mM DTT using PDKtide substrate (0.1 mg/ml) and 20 μM ATP. Reaction was done at 30˚C for 45 min. Amount of ATP transferred was measured using Kinase-Glo® reagent (Promega)
Formulation:
40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Yang, KJ., et al., J Biol Chem. 2008 Jan 18,283(3):1480-91.
2. Kikani, CK., et al., J Cell Biochem. 2005 Dec 15,96(6):1157-62.

Application Reference:
PDK1 controls upstream PI3K expression and PIP3 generation (2014)

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Serine-Threonine
Product Type:
Recombinant Protein
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Product Datasheets

40080_Lot90610.pdf