PDK1, GST-tag Recombinant

Catalog #

40080

$355 *

Size: 10 µg

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Description

Human PDK1, also known as 3-phosphoinositide-dependent protein kinase 1, GenBank Accession No. NM002613, a.a 1-556(full length) with N-terminal GST-tag, expressed in a Baculovirus infected Sf9 cell expression system MW=89 kDa.

This product has been cited 2 times.

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Synonyms

PRO0461; PDPK1; MGC20087; MGC35290; PkB-like; PkB-like 1 ,

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations2

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥84%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol.

89 kDa

Amino Acids

1-556(full length)

Specific Activity

310 pmol/min/µg

Genbank #

NM_002613

UniProt #

O15530

Tag(s)

N-terminal GST-tag

Background

PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns(3,4,5)P3 or PtdIns(3,4)P2. PDK1 then activates protein kinase B (PKB) which, in turn, inactivates glycogen synthase kinase-3 (GSK3). The phosphorylation of other proteins by PKB and GSK3 is likely to mediate many of the intracellular actions of insulin. Thus, PDK1 plays a key role in mediating many of the actions of the second messenger(s) PtdIns(3,4, 5)P3 and/or PtdIns(3,4)P2. The human PDK1 is a 556-residue monomeric enzyme comprising of a catalytic domain that is most similar to the PKA, PKB and PKC subfamily of protein kinases.

References

1. Yang, KJ., et al., J Biol Chem. 2008 Jan 18,283(3):1480-91.
2. Kikani, CK., et al., J Cell Biochem. 2005 Dec 15,96(6):1157-62.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was done in Kinase buffer containing 0.2 mM DTT using PDKtide substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40080 Lot 151111-G2

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