PDK3 Kinase Assay Kit

Catalog #

78286

$1,920 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The PDK3 Kinase Assay Kit is designed to measure PDK3 activity for screening and profiling applications using ADP-Glo® as a detection reagent. The signal is measured using a luminometer or a microplate reader capable of reading chemiluminescence.

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Synonyms

Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial; Pyruvate dehydrogenase kinase isoform 3; PDK-3; CMTX6, GS1-358P8.4

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The PDK3 Kinase Assay Kit comes in a convenient 96-well format, with enough purified PDK3, Casein, ATP, and kinase assay buffer for 96 enzyme reactions.

Materials Required But Not Supplied

ADP-Glo® Kinase Assay (ADP-Glo reagent and Kinase Detection reagent) (Promega #V6930)
Dithiothreitol (DTT 0.5 M)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
100927	PDK3*	50 µg	-80°C
79334	5x Kinase assay buffer	1.5 ml	-20°C
79686	ATP (500 µM)	100 µl	-20°C
	Casein (10 mg/ml)	250 µl	-20°C
79696	96-well plate, white	1	Room Temp.

* The initial concentration of enzyme is lot-specific and will be indicated on the tube containing the protein.

UniProt #

Q15120.1

Background

The pyruvate dehydrogenase (PDH) complex is a mitochondrial complex that converts pyruvate to acetyl-CoA. It is a primary regulator of glucose metabolism by providing the link between glycolysis and the tricarboxylic acid cycle. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, in which phosphorylation results in inactivation of PDH. PDK3 inhibits the PDH complex by phosphorylation of the E1 alpha subunit.

PDK3 participates in the metabolic switch of cancer cells; although its significance in tumor malignancy is unknown. PDK3 has been implicated in drug resistance following the treatment of leukemia or colon cancer.

References

1. Feng, Lan, et al. "miR-497-5p inhibits gastric cancer cell proliferation and growth through targeting PDK3." Bioscience Reports9 (2019): BSR20190654.
2. Lu, Chun-Wun, et al. "Overexpression of pyruvate dehydrogenase kinase 3 increases drug resistance and early recurrence in colon cancer." J. Pathol. 179.3 (2011): 1405-1414.
3. Mohammad, Taj, et al. "Identification of high-affinity inhibitors of pyruvate dehydrogenase kinase-3: Towards therapeutic management of cancer." Biomolec. Struct. Dynam. 39.2 (2021): 586-594.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Instructions for Use

See assay protocol for detailed instructions

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only

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PDK3 Kinase Assay Kit

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