AKT1 Assay Kit

Catalog #

78038

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The AKT1 Assay Kit is designed to measure AKT1 activity for screening and profiling applications using Kinase-Glo® Kinase Assay as a detection reagent.

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Synonyms

Protein Kinase B Alpha assay kit, PKBα assay kit, AKT

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The AKT1 Assay Kit comes in a convenient 96-well format, with enough purified recombinant AKT1 enzyme, AKT1 Substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo® Kinase Assay (Promega #V6071)
Dithiothreitol (DTT, 0.5 M)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog Number	Reagent	Amount	Storage
40003	AKT1	10 µg	-80°C	Avoid multiple freeze/ thaw cycles!
79334	5x Kinase assay buffer 1	1.5 ml	-20°C
79686	ATP (500 μM)	100 µl	-20°C
	AKT1 Substrate (5 mg/ml)	100 µl	-20°C
79696	96-well plate, white	1	Room Temp

UniProt #

P31749

Background

AKT1, also known as Protein Kinase B Alpha (PKBα), is a serine/threonine kinase found in various cell types throughout the body, where it plays a critical role in regulating cell survival, insulin signaling, angiogenesis and tumor formation as well as controlling apoptosis. Importantly, the signaling pathway involving AKT1 kinase appears to be essential for the normal development and function of the nervous system. Dysregulation of Akt1 contributes to a number of serious diseases, including cancer, diabetes, cardiovascular and neurological diseases.