PDE3A (Mouse), GST-Tag Recombinant

Catalog #

60036

$455 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Mouse PDE3A (GenBank Accession No. NM_018779), amino acids 484-1141 end), with N-terminal GST tag, MW = 100 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

PDE3A, phosphodiesterase 3A, PDE3, PDE3-A, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PDE3A1 (Mouse) inhibition curve using a fluorescence polarization assay (#79606). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Mouse

Host Species/Expression System

Sf9 insect cells

Purity

≥70%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 18 mM Glutathione

100 kDa

Amino Acids

484-1141

Specific Activity

≥ 280 pmol/min/µg

Genbank #

NM_018779

Tag(s)

N-terminal GST-tag

References

1. Hung, S.H., et al. J. Biol. Chem. 281 (39), 29236-29244 (2006)

2. Hambleton, R., et al. J. Biol. Chem. 280 (47), 39168-39174 (2005)

Storage/Stability

At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE3A (Mouse) at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only