PDE3A (Dog), FLAG-Tag Recombinant

Catalog #

100264

$470 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Dog PDE3A, also known as Phosphodiesterase 3A, CGMP-Inhibited 3',5'-Cyclic Phosphodiesterase A, and CGI-PDE A, GenBank Accession No. XP_005637137, a.a. 343-998 (end), with C-terminal FLAG-tag, expressed in a HEK293 expression system, MW = 75 KDa.

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Synonyms

Phosphodiesterase 3A, cGMP-Inhibited 3',5'-Cyclic Phosphodiesterase A, and CGI-PDE A, PDE3-A, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Dog

Host Species/Expression System

HEK293

Purity

≥70%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3mM DTT and 20% glycerol.

75 kDa

Amino Acids

343-998 (end)

Genbank #

XP_005637137

Tag(s)

FLAG-Tag

References

1. Hung, S.H., et al. J. Biol. Chem. 281 (39), 29236-29244 (2006)
2. Hambleton, R., et al. J. Biol. Chem. 280 (47), 39168-39174 (2005)

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 μM cAMP, 0.02% TritonX, and serial dilutions of PDE3A at 37ºC for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Absorbance was measured at 620 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Warnings

Avoid multiple freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only