PDE2A V1 (Mouse), GST-tag Recombinant

Catalog #

60017

$455 *

Size: 5 μg

Qty

Bulk/Custom

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Description

Murine PDE2A, variant 1 (GenBank Accession No. NM_001143848), a.a 2-939 (end), with N-terminal GST tag, MW=132 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

PDE2-A, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PDE2A (Mouse) inhibition curve using a fluorescence polarization assay (#79648). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Mouse

Host Species/Expression System

Sf9 Insect Cells

Purity

≥86%

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20 and 20% glycerol.

130 kDa

Amino Acids

2-939 (end)

Specific Activity

≥20,000 pmole/min/µg.

Genbank #

NM_001143848

Tag(s)

N-terminal GST-tag

References

1. Sadhu, K., et al., JHC 47(7), 889-894 (1999).

2. Iffland, A., et al., Biochemistry 44 (23), 8312-8325 (2005).

Storage/Stability

At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE2A V1 (Mouse) at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Scientific Category

PDE

Regulatory Status

Research Use Only