PDE6C, GST-Tag Recombinant

Catalog #

60062

$455 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Recombinant human PDE6c (phosphodiesterase 6C), full length encompassing amino acids 2-858(end). This construct contains an N-terminal GST-tag. A short peptide linker placed between GST and the protein includes a Thrombin Cleavage Site to facilitate removal of the GST portion. The recombinant protein was affinity purified.

This product has been cited 13 times.

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Synonyms

PDE6C, phosphodiesterase 6C, PDE6-C, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PDE6C inhibition curves using a fluorescence polarization assay (#79501). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations13

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥5%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCL, 3 mM DTT, 20% Glycerol and 2 mM Glutathione.

125 kDa

Amino Acids

full length

Specific Activity

≥ 3,000 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.

Genbank #

NM_006204

UniProt #

P51160

Tag(s)

N-terminal GST-tag

References

1. Martinez S.E. et al., J Biol Chem. 2008 Sep 19;283(38):25913-9.
2. Stearns G. et al., J Neurosci. 2007 Dec 12;27(50):13866-74.

Storage/Stability

>6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cGMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE6C at 37°C for 60 min. Quantified by 5'- nucleotidase cleaving the 5' -GMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Notes

This product is a more active version of former BPS Cat. #60060.

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only

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Datasheets/Protocols

60062_Lot_171023-A.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

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PDE6C, GST-Tag Recombinant

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