PDE2A (Mouse) Assay Kit

Catalog #
79648
$405 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The Mouse PDE2A Assay Kit is designed for identification of inhibitors of Mouse PDE2A using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Mouse PDE2A to the binding agent.

The key to the Mouse PDE2A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Mouse PDE2A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Mouse PDE2A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

Need us to run inhibitor screens or profile your compounds against PDE2A (Mouse)? Check out our Phosphodiesterase Screening Services.

Synonyms
inhibitor screening, assay kit, PDE2A mouse, PDE Assay kit, PDE2-A, PDE
Product Info
Storage and Usage
Citations
Assay Kit Format
Fluorescence Polarization
Species
Mouse
Supplied As
The Mouse PDE2A Assay Kit comes in a convenient 96-well format, with purified Mouse PDE2A enzyme, fluorescently labeled substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied

Fluorescent microplate reader capable to measure fluorescence polarization.

Adjustable micropipettor and sterile tips.

1,4-Dithiothreitol (DTT) 1 M in anhydrous DMSO.

Format

COMPONENTS:

Background
Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cGMP signaling. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light.
References

1. Salpietro, V., Perez‐Dueñas B., Nakashima K., et al. A homozygous loss‐of‐function mutation in PDE2A associated to early‐onset hereditary chorea. Movement Disorders. 2018; 33(3):482-488.

2. Gomez, L., Massari, M., et al. Design and Synthesis of Novel and Selective Phosphodiesterase 2 (PDE2a) Inhibitors for the Treatment of Memory Disorders. J. Med. Chem. 2017; 60(5):2037-2051.