RARβ Luciferase Reporter HEK293 Cell Line

Catalog #

60603

$1,895 *

Size: 2 vials

Qty

Bulk/Custom

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Description

RARβ Luciferase Reporter HEK293 Cell Line is a HEK293 cell line engineered to express firefly luciferase, under the control of retinoic acid response elements, and with full length human Retinoic Acid Receptor beta (RARb) (accession #P10826-2). RARβ Luciferase Reporter HEK293 Cell Line is designed for monitoring the activity of RARb. This cell line was functionally validated by stimulation with all-trans retinoic acid.

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Figure 1: Illustration of the mechanism of action of RARγ Luciferase Reporter HEK293 Cell Line. Upon activation with retinoic acid, RAR dimerizes with RXR, and activates RARE and luciferase expression. The level of activation corresponds directly to the luciferase signal.

Interested in screening and profiling inhibitors or activators of Retinoic Acid Receptor beta without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

RAR beta, Retinoic acid receptor beta, RARB

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Product Data Gallery

Dose response curve of RARβ Luciferase Reporter HEK293 Cell Line to all-trans retinoic acid (ATRA)

Product Info

Storage and Usage

Citations

Host Cell Line

HEK293, Human Embryonic Kidney, epithelial-like cells, adherent

Supplied As

Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 6	BPS Bioscience #60183
Growth Medium 6A	BPS Bioscience #79542

Materials Used in the Cellular Assay

Name	Ordering Information
ATRA	Sigma #R2625
Assay Medium 6A	BPS Bioscience #82211
96-well tissue culture treated white clear-bottom assay plate	Corning #3610
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

Biological Activity

This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid. The expression of RARβ is confirmed by Western blotting.

Genbank #

P10826-2

UniProt #

P10826-2

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Retinoic acid receptor (RAR) belongs to the family of nuclear receptors and has three subtypes, RARa, RARb, and RARg. RAR heterodimerizes with retinoic X receptor (RXR) and acts as a transcription factor that regulates the growth and differentiation of both normal and malignant cells. When RAR binds to its ligands, all-trans retinoic acid or 9-cis retinoic acid, RAR/ RXR heterodimer binds to retinoic acid response elements in the promoter region of target genes and recruits coactivator proteins, leading to transcription and expression of the downstream target genes. RARβ dysfunction can result in cervical carcinoma. Abnormal promoter DNA hypermethylation has also been linked to cancer.

References

Petkovich M., et al., 1987 Nature 330(6147): 444-450.
Allenby G., et al., 1993 Proc. Natl. Acad. Sci. USA 90(1): 30-34

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 6 (BPS Bioscience #60183): DMEM medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 6A (BPS Bioscience #79542): DMEM medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 400 µg/ml of Geneticin (G418), 1 µg/ml of Puromycin, and 100 µg/ml Hygromycin B.

Media Required for Functional Cellular Assay 
Assay Medium 6A (BPS Bioscience #82211): Phenol red-free DMEM, 10% Charcoal Stripped FBS, and 1% Penicillin/Streptomycin.

Instructions for Use

See cell line data sheet for detailed culturing and assay protocol.

Applications

Monitor RARb-regulated pathway activity.
Screen for agonists or antagonists of RARb.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Nuclear Receptor

Regulatory Status

Research Use Only