ARE Luciferase Reporter HepG2 Cell Line (Nrf2 Antioxidant Pathway)

Catalog #

60513

$2,570 *

Size: 2 vials

Qty

Bulk/Custom

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Description

The antioxidant response element (ARE) Luciferase Reporter HepG2 hepatic cell line contains a stably integrated firefly luciferase reporter under the control of ARE, which is recognized by the transcription factor Nuclear factor erythroid 2-related factor 2 (Nrf2). Luciferase expression correlates with activation of Nrf2 and the antioxidant pathway. This cell line was validated by stimulation with tert-butylhydroquinone, sulforaphane, and RTA-408 (omaveloxone).

Figure 1: Illustration of the ARE Luciferase Reporter HepG2 Cell Line (Nrf2 Antioxidant Pathway) mechanism.

Interested in screening and profiling inhibitors or activators of the Nrf2 signaling pathway without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

This product has been cited 44 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

ARE cell line, Nrf2 reporter, Nrf, Hepg2, ARE

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Product Data Gallery

ARE Luciferase Reporter HepG2 cells response to sulforaphane (3 µM) and tert-butylhydroquinone (160 µM).

Dose response of sulforaphane in ARE Luciferase Reporter HepG2 cells. The results are shown as fold induction of unstimulated luciferase activity.

Dose response of ARE Luciferase Reporter HepG2 cells to RTA-408.

Product Info

Storage and Usage

Citations44

Host Cell Line

HepG2, Human hepatocellular carcinoma, epithelial-like cells, adherent

Supplied As

Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1k	BPS Bioscience #79533

Materials Used in the Cellular Assay

Name	Orderinig Information
Tert-butylhydroquinone	Signma #112941
DL-Sulforaphane	Sigma #S4441
RTA-408 (Omaveloxolone)	MedChemExpress #HY-12212
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
White clear-bottom 96-well cell culture plate
Luminometer

UniProt #

Q16236

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Nrf2 is a transcription factor activated in response to toxic and oxidative drugs and chemicals. Under normal conditions, Nrf2 is retained in the cytosol through its binding to the cytoskeletal protein Kelch-like ECH-associated protein 1 (Keap1). Nrf2 is released from Keap1 upon oxidative stress and translocates to the nucleus, where it binds to the ARE promoter region of genes involved in the oxidative stress response and in drug detoxification, including genes encoding antioxidant enzymes that protect the cell from oxidative damage. Nrf2 supports cellular resistance to carcinogens and to inflammation, and dysfunction can lead to neurological diseases such as Alzheimer disease, Parkinson’s and Huntington’s diseases and amyotrophic lateral sclerosis (AMS). Its function is particularly important in the liver, where it contributes to protection against viral hepatitis and alcoholic and nonalcoholic liver disease. Molecules able to interact with Nrf2 and enhance is protective role are of great interest in medicine.

References

Cuadrado A, et al. 2019 Nature Rev. Drug Discovery, 18: 295-317.
Zgorzynska E., et al., 2021 Int J Mol Sci, 22(17): 9592.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture

Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na-pyruvate, 1% Penicillin/Streptomycin.

Growth Medium 1K (BPS Bioscience #79533): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na-pyruvate, 1% Penicillin/Streptomycin and 600 µg/ml of Geneticin.

Media Required for Functional Cellular Assay

Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na-pyruvate, 1% Penicillin/Streptomycin.

Instructions for Use

See data sheet for detailed culturing and assay protocol.

Applications

Monitor the Nrf2 antioxidant response pathway.
Screen for compound activity on the Nrf2 antioxidant response pathway

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antioxidant Signaling pathway

Regulatory Status

Research Use Only