ARE reporter cell line (Nrf2 antioxidant pathway)

Catalog #: 60513
Size: 2 vials
The Nrf2 antioxidant response pathway plays an important role in cellular antioxidant defense. Nrf2, a basic leucine zipper transcription factor, induces the expression of antioxidant and phase II enzymes by binding to the ARE (antioxidant response element) region of the gene promoter. Under basal conditions, Nrf2 is retained in the cytosol by binding to the cytoskeletal protein Keap1. Upon exposure to oxidative stress or other ARE activators, Nrf2 is released from Keap1 and translocates to the nucleus, where it can bind to the ARE, leading to the expression of antioxidant and phase II enzymes that protect the cell from oxidative damage.
The ARE Reporter – Hep G2 cell line is designed to monitor Nrf2 antioxidant response pathway. The cell contains a firefly luciferase gene under the control of ARE (antioxidant response element) stably integrated into Hep G2 cells.
Materials Required But Not Provided:

• Tert-butylhydroquinone (Sigma # 112941). Prepare stock solution in DMSO.
• DL-Sulforaphane (Sigma # S4441). Prepare stock solution in DMSO.
Assay medium: Thaw Medium 1 (BPS Cat. #60187)
Growth Medium 1K (BPS Cat. #79533)
• 96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate
ONE-Step™ Luciferase Assay System (BPS Cat. #60690)
• Luminometer

ARE cell line, Nrf2 reporter, Nrf, Hepg2, ARE
Supplied As:
Each vial contains ~2 X 106 cells in 1 ml of 10% DMSO.
Biological Activity:
This cell line is validated for the response to the stimulation of tert-butylhydroquinone and sulforaphane.
Host Cell line:
Hep-G2 cells
Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 1 (BPS Cat. #60187): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Life Technologies #26140-079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na-pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01).

Growth Medium 1K (BPS Cat. #79533): Thaw Medium 1 (BPS Cat. #60187) and 600 µg/ml of Geneticin (Life Technologies #11811031).

Aqueous solution containing DMSO.
Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:

Store in liquid nitrogen immediately upon receipt.

• Monitor Nrf2 antioxidant response pathway activity
• Screen for activators or inhibitors of the Nrf2 antioxidant response pathway

1. Lee, JM et. al. (2004) An important role of Nrf2-ARE pathway in the cellular defense mechanism. J Biochem Mol Biol. 37(2): 139-143.
2. Dinkova-Kostova, AT (2002) Direct evidence that sulfhydryl groups of Keap1 are the sensors regulating induction of phase 2 enzymes that protect against carcinogens and oxidants. Proc Natl Acad Sci U S A. 99(18): 11908-11913.

Application Reference(s):
1. Hüpotermia mõju antioksüdatiivse süsteemi induktsioonile (2017)
2. The role of plant processing for the cancer preventive potential of Ethiopian kale (Brassica carinata) (2017)
3. Regulation of Gene Expression by the Cd44-Intracytoplasmic Domain (CD44-ICD) (2017)
4. Design, Synthesis, and Biological Activities of Vibsanin B Derivatives: A New Class of HSP90 C-Terminal Inhibitors (2017) 
5. Activation of anti-oxidant Nrf2 signaling by substituted trans stilbenes (2017)


License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Avoid freeze/thaw cycles
Scientific Category:
Antioxidant Signaling pathway
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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