LSD2 (KDM1B), GST-Tev-Tag Recombinant

Catalog #

50124

$475 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Recombinant human LSD2 (lysine demethylase 1B, KDM1B), encompassing amino acids 2-822 (end). In this construct the N-terminal end of the protein is fused to GST via a short peptide linker placed between GST consisting of an optimal TEV protease target sequence that allows cleavage of GST (TEV: tobacco etch virus cysteine protease BPS Bioscience #50308). The recombinant protein was affinity purified.

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Synonyms

KDM1B, AOF1, LSD2, C6orf193

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

LSD2 (KDM1B) (Dimethyl) inhibition curve using an AlphaLISA assay (#50614). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

LSD2 (KDM1B) (GST-TEV-2-822(end))

Host Species/Expression System

Sf9 insect cells

Purity

≥70%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 16 mM glutathione

121 kDa

Amino Acids

2-822(end)

Specific Activity

11 pmol/min/µg

Genbank #

NM_001364614

UniProt #

Q8NB78

Tag(s)

N-terminal GST-Tev Tag

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

For standard, various concentrations of H3, 1-21 K4 (1Me)-Biotin were added to the plate. For test lots, reaction mixture contained 2MeK4, diluted primary antibody 22, 1x LSD2 buffer, and various amounts of LSD2 enzyme—incubated for 1 hour at room temperature. Followed by addition of anti-rabbit acceptor beads and streptavidin-conjugated donor beads.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Demethylase

Regulatory Status

Research Use Only