Anti–LSD2 (KDM1B) polyclonal antibody

Catalog #

25299

$335 *

Size: 100 µl

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Polyclonal antibody raised in rabbit against human KDM1B (lysine (K)–specific demethylase 1B) using two KLH–conjugated synthetic peptides containing a sequence from the N–terminal and the central region of the protein, respectively.

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Synonyms

AOF1, LSD2, C6orf193

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Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Whole serum

Format

aqueous solution

Formulation

Whole antiserum from rabbit containing 0.05% azide

Concentration

100 µl

Immunogen

synthetic peptide

Background

KDM1B (UniProtKB/Swiss–Prot entry Q8NB78), also known as LSD2, is a histone demethylase that demethylates lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. KDM1B is able to demethylate both mono– and di–methylated lysine 4 of histone H3, but has no effect on tri–methylated lysine 4, mono–, di– or tri–methylated lysine 9, mono–, di– or tri–methylated lysine 27, mono–, di– or tri–methylated lysine 36 of histone H3, or on mono–, di– or tri–methylated lysine 20 of histone H4. KDM1B is also required for de novo DNA methylation of a subset of imprinted genes during oogenesis. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human KDM1B (Cat. # 25299). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:14,250.

Western blot analysis using the antibody directed against KDM1B
Nuclear extracts (25 µg) from K562 cells were analyzed by Western blot using the antibody against KDM1B (Cat. # 25299) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size 92 kDa) is indicated on the right; the marker (in kDa) is shown on the left.

Applications

ELISA (1:500)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–LSD2 (KDM1B) polyclonal antibody

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