LSD1 (KDM1A), Variant 2 Recombinant

Catalog #

50097

$450 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human LSD1, also known as KDM1A (lysine (K)-specific demethylase 1A) or AOF2, amine oxidase (flavin containing) domain 2), GenBank Accession No. NM_015013, a.a. 158-end, expressed in E. coli expression system. MW=78 kDa.

This product has been cited 19 times.

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Synonyms

KDM1A, AOF2, lysine (K)-specific demethylase 1A, amine oxidase (flavin containing) domain 2, LSD

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

LSD1 (KDM1A), Variant 2

Product Info

Storage and Usage

Citations19

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

100mM Na2HPO4/NaH2PO4, pH 7.4

78 kDa

Amino Acids

158–end

Specific Activity

20.6 pmol/min/µg

Genbank #

NM_015013

UniProt #

O60341

References

1. Lan, F., et al., Nature. 448, 7154, 718-722, 2007.
2. Forneris, F., et al., J. Biol. Chem. 282, 28, 20070-20074, 2007.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was performed according to BPS catalog #50109 with LSD1 titrated from 400 ng/rxn – 0 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Notes

Important: If you intend to assess LSD1 activity in an assay that analyzes hydrogen peroxide, such as BPS Catalog #50106 and 50107, please include a request for "Peroxidase Rxn Grade LSD1."

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Demethylase

Regulatory Status

Research Use Only