LAG3, His-Tag, HiP™ Recombinant

Catalog #

71229

$320 *

Size: 50 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Human secreted LAG3, also known as Lymphocyte-Activation Gene 3 and CD223. GenBank Accession No. NM 002286, a.a. 23-450 expressed in a HEK293 cell expression system with C-terminal His-Tag. MW = 48 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

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Synonyms

Lymphocyte-Activation Gene 3, CD223, Lag, Lag-3

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Gel Filtration Curve

LAG3:FGL1[Biotinylated] inhibition curve using a chemiluminescent assay (#79789). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

HEK 293 cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

20 mM NaOAC, pH 5.5, 500 mM NaCl, 0.02% Tween 20 and 10% glycerol.

48 kDa

Amino Acids

23-450

Genbank #

NM_002286

UniProt #

P18627

Tag(s)

C-terminal His-Tag

References

1. Byrnes, et al.. Cytometry A. 2012 Sep;81(2):165-8.
2. Di, Carlo., et al., J. Pathol. 2005 Jan; 205(1):82-91.

Storage/Stability

At least 6 months at –80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying protein binding and screening small molecules.

Shipping Temperature

-80°C (dry ice)

Notes

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

71229 Lot 160229.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

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LAG3, His-Tag, HiP™ Recombinant

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