FGL1:LAG3 TR-FRET Assay Kit

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Catalog #

79739

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Description

The FGL1:LAG3 TR-FRET Assay is designed to measure the inhibition of LAG3 binding to FGL1 in a homogeneous 96 or 384 reaction format. This TR-FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated LAG3, His-tagged FGL1 protein, and an inhibitor are incubated for one hour. Then, anti-His Tb donor and dye-labeled acceptor are added and fluorescence intensity is measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against FGL1:LAG3? Check out our Immunotherapy Biochemical Screening Services.

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Synonyms

HFREP1, HP-041, LFIRE-1, LFIRE1, fibrinogen like 1

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

TR-FRET

Materials Required But Not Supplied

Fluroescence microplate reader capable of measuring Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips

Format

96 Reactions:

Catalog Number	Component	Amount	Storage
100330	FGL1, His tag	5 µg	-80°C	Avoid multiple freeze/thaw cycles!
71147	LAG3 (CD223), Biotin-labeled (Human) HiP™	10 µg	-80°C
30017	Anti-His Tb Donor	2 x 10 µl	-20°C
	Dye-labeled Acceptor	2 x 10 µl	-20°C
	3x FGL1 TR-FRET Buffer	4 ml	-20°C
79696	White, 96-well microtiter plate	1	Room. temp

384 Reactions:

Catalog Number	Component	Amount	Storage
100330	FGL1, His tag	2 x 5 µg	-80°C	Avoid multiple freeze/thaw cycles!
71147	LAG3 (CD223), Biotin-labeled (Human) HiP™	2 x 10 µg	-80°C
30017	Anti-His Tb Donor	2 x 10 µl	-20°C
	Dye-labeled Acceptor	2 x 10 µl	-20°C
	3x FGL1 TR-FRET Buffer	4 ml	-20°C
	White, 384-well microtiter plate	1	Room. temp

Background

Lymphocyte-activation gene 3 (LAG3, also CD223) is a cell surface receptor that negatively regulates activation and proliferation of T cells. Fibrinogen-like protein 1 (FGL1), a liver-secreted protein, is a functional LAG3 ligand. Blockade of the FGL1-LAG3 interaction is implicated in promoting antitumor immunity.

References

1. Wang, J., et al. Cell 2019, 176(1-2): 334-347
2. Visan. I., et al. Nature Immunol. 2019, 20(2): 111

Storage/Stability

Up to 6 months from date of receipt, when stored as recommended.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Warnings

Avoid multiple freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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FGL1:LAG3 TR-FRET Assay Kit

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