FGL1:LAG3 Inhibitor Screening Assay Kit

Catalog #

78824

$1,025 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The FGL1:LAG3 Inhibitor Screening Assay is an AlphaLISA®-based assay designed to measure the inhibition of LAG3 binding to FGL1 in a homogeneous 384-reaction format. The assay is straightforward: first, FGL1 and LAG3 are incubated with or without a compound of interest. Then, acceptor and donor beads are added to the reaction, followed by reading the Alpha-counts.

Illustration of the assay principle.
FGL1 contains a His tag recognized by the Nickel chelate acceptor bead, whereas LAG3 is biotinylated, allowing its binding to the streptavidin-coated acceptor bead. Interaction between FGL1 and its receptor LAG3 brings the donor and acceptor beads in proximity. A singlet oxygen generated upon excitation of the donor bead leads to the excitation of the acceptor bead, which emits light. Light emission in the assay is proportional to the level of interaction. AlphaLISA™ immunoassays are a no-wash alternative to ELISA. These assays are robust, highly amenable to high throughput applications, and ideal for a minimal hands-on approach.

Need us to run inhibitor screens or profile your compounds against FGL1:LAG3? Check out our Immunotherapy Biochemical Screening Services.

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Synonyms

Fibrinogen-like protein 1, HP-041, Hepassocin, HPS, Hepatocyte-derived fibrinogen-related protein 1, HFREP-1, Liver fibrinogen-related protein 1, LFIRE-1, HFREP1

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Product Data Gallery

Inhibition of FGL1:LAG3 interaction using a neutralizing anti-LAG3 antibody

Product Info

Storage and Usage

Citations

Assay Kit Format

AlphaLISA®

Supplied As

The FGL1:LAG3 Inhibitor Screening Assay Kit contains sufficient amounts of purified recombinant FGL1 and LAG3 proteins, and assay buffer for 400 reactions.

Materials Required But Not Supplied

AlphaLISA® Nickel Chelate acceptor beads, 5 mg/ml PerkinElmer #AL108C
AlphaScreen® Streptavidin donor beads, 5 mg/ml PerkinElmer #6760002S
Optiplate - 384 PerkinElmer #6007290
AlphaScreen® microplate reader
Adjustable micropipettor and sterile tips

Format

Catalog #	Name	Amount	Storage
	FGL1, His Tag	10 µg	-80°C
71147	LAG3 (CD223), Biotin-Labeled (Human) HiP™	32 µg	-80°C
79311	3x Immuno Buffer	4 ml	-20°C

UniProt #

Q08830

Background

Lymphocyte-activation gene 3 (LAG3, also known as CD223) is a cell surface receptor that negatively regulates the activation and proliferation of T cells. Fibrinogen-like protein 1 (FGL1), a liver-secreted protein present in the plasma, is a functional LAG3 ligand. Blockade of the FGL1-LAG3 interaction has been proposed as a therapeutic strategy to promoting antitumor responses in cancer patients.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Screen for inhibitors of LAG3 binding to FGL1.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Contraindications

Green and blue dyes, such as Trypan Blue, absorb light in the AlphaScreen™ signal emission range (520-620 nm). Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen™ assays.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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FGL1:LAG3 Inhibitor Screening Assay Kit

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