GR-GAL4 Reporter (Luc)-HEK293 Recombinant Cell Line (Glucocorticoid Receptor Pathway)

Catalog #: 60655
Size: 2 vials
Species: Human
Host Species: HEK293 Cells
The glucocorticoid signaling pathway plays an important role in development, fluid homeostasis, cognition, immune response, and metabolism. Glucocorticoids are a class of steroid hormones that bind to the glucocorticoid receptor, causing it to translocate to the nucleus. Upon translocation, the receptor can regulate the transcription of a large number of genes, including those that regulate glucose metabolism and inflammatory responses.
The Glucocorticoid Receptor Pathway GAL4 Reporter (Luc) – HEK293 Cell Line contains a firefly luciferase gene under the control of glucocorticoid receptor ligand binding domain that is fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR) stably integrated into HEK293 cells. This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. This cell line is validated for response to stimulation of dexamethasone and to the treatment with mifepristone, an inhibitor of the glucocorticoid signaling pathway.
Materials Required But Not Provided:

Materials Required but Not Supplied for Cell Culture

Materials Required but Not Supplied for Cellular Assay

  • Dexamethasone (Sigma #D4902)
  • Mifepristone/RU-486 (Sigma #M8046)
  • HEK293 assay medium: DMEM (without phenol red) (Hyclone #SH30604.01) + 10% charcoal/dextran-treated FBS (Hyclone #SH30068.02), 4 mM L-Glutamine + 1% Pen/Strep
  • 96-well tissue culture treated white clear-bottom assay plate (Corning #3610)
  • ONE-Step™ Luciferase Assay System (BPS Cat. #60690)
  • Luminometer
GAL4, Glucocorticoid Receptor, Galectin
Supplied As:
Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO.
Biological Activity:
This cell line is validated for the response to the stimulation of dexamethansone and to the treatment of the inhibitor mifepristone.
Host Cell line:
HEK293 Cells
Mycoplasma Testing:
The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 1 (BPS Cat. #60187): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Invitrogen #26140-079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01)

Growth Medium 1G (BPS Cat. #79544)Thaw Medium 1 (BPS Cat #60187) and 400 µg/ml of Geneticin (Life Technologies #11811031) and 50 µg/ml of Hygromycin B (Hyclone #SV3007001).

Storage / Stability:
Immediately upon receipt, store in liquid nitrogen.
Monitor glucocorticoid signaling pathway activity and screen activators or inhibitors of the glucocorticoid signaling pathway.

Paguio A, et al. (2010) Improved Dual-Luciferase Reporter Assays for Nuclear Receptors. Curr Chem Genomics. 4: 43-49.


License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Scientific Category:
Cell Signaling Pathway
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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