GR-GAL4 Luciferase Reporter HEK293 Cell Line (Glucocorticoid Receptor Pathway)

Catalog #

60655

$10,500 *

Size: 2 vials

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The GR-GAL4 Luciferase Reporter HEK293 Cell Line is a HEK293 cell line expressing firefly luciferase under the control of glucocorticoid receptor ligand binding domain fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR). This fusion construct activates firefly luciferase expression via control of a multimerized GAL4 upstream activation sequence (UAS). This system allows specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and has low cross-reactivity with other nuclear receptor pathways. This cell line has been validated by stimulation with dexamethasone and treatment with mifepristone, an inhibitor of the glucocorticoid signaling pathway.

Interested in screening and profiling inhibitors or activators of glucocorticoid receptor without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

●

Synonyms

GAL4, Glucocorticoid Receptor, Galectin

●

Product Data Gallery

Dose response curve of GR-GAL4 Luciferase Reporter HEK293 Cell Line to dexamethasone

Dose response curve of GR-GAL4 Luciferase Reporter HEK293 Cell Line dexamethasone-induced-reporter activty to mifepristone

Product Info

Storage and Usage

Citations

Host Cell Line

HEK293, Human Embryonic Kidney, epithelial-like cells, adherent.

Species

Human

Supplied As

Each vial contains ≥1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1G	BPS Bioscience #79544

Materials Required for Cellular Assay

Name	Ordering Information
Dexamethasone	Sigma #D4902
Mifepristone/RU-486	Sigma #M8046
96-well tissue culture treated white clear-bottom assay plate	Corning #3610
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

Biological Activity

This cell line is validated for the response to the stimulation of dexamethansone and to the treatment of the inhibitor mifepristone.

UniProt #

P04150

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The glucocorticoid signaling pathway plays an important role in development, fluid homeostasis, cognition, immune response, and metabolism. Glucocorticoids are a class of steroid hormones that bind to the glucocorticoid receptor, causing it to translocate to the nucleus. Upon translocation, the receptor can regulate the transcription of a large number of genes by binding to the GRE (glucocorticoid response elements), including those that regulate glucose metabolism and inflammatory responses. The complex receptor:ligand results in up-regulation of expression of anti-inflammatory proteins (transactivation), and downregulates the expression of pro-inflammatory ones by inhibiting the translocation of the transcription factors required for their expression to the nucleus (transrepression). Glucocorticoids are used in the treatment of diseases linked to over stimulation of the immune system, for example in allergies, asthma, autoimmune diseases, and sepsis. They have also been used in cancer therapy at high doses, as they inhibit lymphocyte proliferation in lymphoma and leukemia, and organ transplant. Long term use of glucocorticoids can result in cataract, hypertension, type 2 diabetes, among other side effects. An in depth understanding of the glucocorticoid signaling pathway will lead to better tailored treatment options.

References

Paguio A, et al., 2010 Curr Chem Genomics. 4: 43-49.

Storage/Stability

Immediately upon receipt, store in liquid nitrogen.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate and 1% Penicillin/Streptomycin

Growth Medium 1G (BPS Bioscience #79544): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin, 400 µg/ml of G418 and 50 µg/ml of Hygromycin B.

Media Required for Functional Cellular Assay
Assay Medium: DMEM (without phenol red) + 10% charcoal/dextran-treated FBS, 4 mM L-Glutamine and 1% Pen/Strep

Applications

Monitor glucocorticoid signaling pathway activity.
Screen for compound activity on the glucocorticoid signaling pathway.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only