GR-GAL4 Luciferase Reporter Jurkat Cell Line

Catalog #

78525

$10,500 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Glucocorticoid Receptor (GR)-GAL4 Luciferase Reporter Jurkat Cell Line contains an engineered transcription factor stably integrated into the genome of Jurkat cells, which consists of the glucocorticoid receptor ligand binding domain fused to the DNA binding domain of GAL4. This fusion construct activates firefly luciferase expression which is under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. This cell line is validated for response to stimulation of dexamethasone and to the treatment with mifepristone, an inhibitor of the glucocorticoid signaling pathway.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of glucocorticoid receptor without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Dose response of Dexamethasone in the GR-GAL4 Luciferase Reporter Jurkat Cell Line

Dose response of Mifepristone in GR-GAL4 Luciferase Reporter Jurkat Cell Line

Product Info

Storage and Usage

Citations

Host Cell Line

Jurkat (clone E6-1), human T lymphoblast, suspension

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience, #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2A	BPS Bioscience #60190

Materials Required for Cellular Assay

Name	Ordering Information
Assay Medium 2C	BPS Bioscience #78544
Dexamethasone	Cayman #11015
Mifefristone	Cayman #10006317
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The glucocorticoid signaling pathway plays an important role in development, fluid homeostasis, cognition, immune response, and metabolism. Glucocorticoids are a class of steroid hormones that bind to the glucocorticoid receptor, causing it to translocate to the nucleus. Upon translocation, the receptor regulates the transcription of a large number of genes, including those that regulate glucose metabolism and inflammatory responses.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture

Thaw Medium 2 (BPS Bioscience #60184): RPMI-1640 medium supplemented with 10% FBS and 1% Penicillin and Streptomycin.

Growth Medium 2A (BPS Bioscience #60190): RPMI-1640 medium supplemented with 10% FBS, 1% Penicillin and Streptomycin plus 1000 µg/ml of G418 and 200 µg/ml of Hygromycin B.

Media Required for Functional Cellular Assay

Assay Medium 2C (BPS Bioscience #78544): RPMI-1640 medium (no phenol red) supplemented with 10% charcoal-stripped FBS and 1% Penicillin and Streptomycin.

Applications

Monitor glucocorticoid signaling pathway activity.
Screen activators or inhibitors of the glucocorticoid signaling pathway.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only