DRAK2, GST-Tag Recombinant

Catalog #

71224

$310 *

Size: 100 μg

Qty

Bulk/Custom

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Description

Human DRAK2, also known as DAP kinase-related apoptosis-inducing protein kinase 2 or death-associated protein kinase-related 2, GenBank Accession No. NM_004226.3, a.a. 2-372 (end), with N-terminal GST-tag, expressed in a baculovirus-infected Sf9 cell expression system. MW=69 kDa.

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Synonyms

DAP kinase-related apoptosis-inducing protein kinase 2 or death-associated protein kinase-related 2

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Product Data Gallery

Specific Activity

4-20% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥75%

Supplied As

Aqueous buffer solution.

Formulation

50 mM HEPES, pH 7.5, 0.001% Brij-35, 1 mM DTT, 10 mM MgCl2, 150 mM KCl, 5% Glycerol, 0.1 mM EDTA.

69 kDa

Amino Acids

2-372 (end)

Specific Activity

15 pmole/min/μg

Genbank #

NM_004226.3

UniProt #

O94768

Tag(s)

N-terminal GST-Tag

References

1. McGargill, M. et al, Immunity. 21 (6): 781-791 (2004).
2. Mao, J. et al, Journal of Biological Chemistry. 281: 12587-12595 (2006).

Storage/Stability

At least 6 months at –80°C. Avoid freeze/thaw cycles.

Assay Conditions

Assay was done in Kinase buffer containing 1 mM DTT using MRCL3 peptide substrate (0.2 mg/ml) 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Scientific Category

Immunotherapy, Serine-Threonine Kinase

Regulatory Status

Research Use Only