DRAK2 Recombinant

Catalog #

71225

$310 *

Size: 100 μg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Human DRAK2, also known as DAP kinase-related apoptosis-inducing protein kinase 2 or death-associated protein kinase-related 2 (GenBank Accession No. NM_004226.3), a.a. 2-372 (end), MW = 43 kDa, expressed in a baculovirus-infected Sf9 cell expression system.

●

Synonyms

DAP kinase-related apoptosis-inducing protein kinase 2, death-associated protein kinase-related 2

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 1 mM DTT, 1 mM EDTA

43 kDa

Amino Acids

2-372 (end)

Genbank #

NM_004226.3

UniProt #

O94768

References

1. McGargill, M. et al, Immunity. 21 (6): 781-791 (2004).

2. Mao, J. et al, Journal of Biological Chemistry. 281: 12587-12595 (2006).

Storage/Stability

At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1-0.5%).

Assay Conditions

Assay was done in Kinase buffer containing 1 mM DTT using MRCL3 peptide substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 50 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega)

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Scientific Category

Immunotherapy, Serine-Threonine Kinase

Regulatory Status

Research Use Only