cKit (D816V), His-tag Recombinant

Catalog #

40252

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human cKit with D816V substitution, GenBank Accession No. NM_000222, a.a. 544-end with N-terminal His- tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=53 kDa.

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Synonyms

KIT, cKIT, c-KIT, PBT, CD117, Piebald Trait, SCFR, Mast/Stem Cell Growth Factor Receptor

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥32%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM Imidazole, 3mM DTT, and 20% Glycerol.

53 kDa

Amino Acids

544–976(end)

Specific Activity

473 pmol/min/μg.

Genbank #

NM_000222

UniProt #

P10721

Tag(s)

N-terminal His-tag

References

1. Schumaker, J.A., et al. (2008) J. Clin. Pathol. 61:109-114.
2. Sun, J. et al. (2009) J. Biol. Chem. 284: 11039-11047.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 1 mM DTT using 0.2 mg/ml Poly-(Glu4:Tyr)-biotin substrate and 20 μM ATP. Reaction was done at 30°C for 30 min. Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40252 Lot 110831.pdf

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cKit (D816V), His-tag Recombinant

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