cKIT (D816V) Assay Kit
The cKIT (D816V) Assay Kit is designed to measure cKIT (D816V) activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator
| Catalog Number |
Reagent |
Amount |
Storage |
|
| 40252 | cKIT(D816V), His-Tag | 10 µg | -80°C |
|
| 79334 | 5x Kinase assay buffer I | 1.5 ml | -20°C | |
| 79686 | ATP (500 μM) | 50 µl | -20°C | |
| 40217 | Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1) (10 mg/ml) |
50 µl | -20°C | |
| 79696 | 96-well plate, white | 1 | Room Temp. | |
c‐KIT is a proto‐oncogene and a type III transmembrane receptor for mast cell growth factor, also known as stem cell factor. It plays an essential role in the regulation of cell survival and proliferation, as well as hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and mast cell development, migration and function. The D816V substitution in the cKIT kinase domain has been identified in peripheral blood mononuclear cells of patients suffering from mastocytosis with an associated hematologic disorder. Gastrointestinal stromal tumors (GIST), acute myeloid leukemia, and germ cell tumors have also been demonstrated to carry this mutation.
1. Schumaker, J.A., et al. (2008) J. Clin. Pathol. 61:109-114.
2. Sun, J. et al. (2009) J. Biol. Chem. 284: 11039-11047.
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