cKIT Assay Kit

Catalog #

79815

$535 *

Size: 96 Reactions

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The cKIT Assay Kit is designed to measure cKIT activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.

This product has been cited 2 times.

●

Synonyms

stem cell factor kit

●

Product Data Gallery

Product Info

Storage and Usage

Citations2

Assay Kit Format

Luminescent

Supplied As

The cKIT Assay Kit comes in a convenient 96-well format, with enough purified recombinant cKIT enzyme, Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1), ATP, and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

ADP-Glo® Kinase Assay (Promega #V6930) Dithiothreitol (DTT, 1 M; optional) Microplate reader capable of reading luminescence Adjustable micropipettor and sterile tips 30°C incubator

Format

Catalog Number	Reagent	Amount	Storage
40250	cKit, His-Tag	20 µg	-80°C	Avoid multiple freeze/ thaw cycles!
79334	5x Kinase Assay Buffer 1	1.5 ml	-20°C
79686	ATP (500 μM)	100 µl	-20°C
40217	Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1) (10 mg/ml)	100 µl	-20°C
79696	96-well plate, white	1	Room Temp.

UniProt #

P10721

Background

c‐KIT is a proto‐oncogene and a type III transmembrane receptor for mast cell growth factor, also known as stem cell factor. It plays an essential role in the regulation of cell survival and proliferation, as well as hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and mast cell development, migration and function. Activating mutations in cKIT are associated with gastrointestinal stromal tumors, testicular seminoma, mast cell disease, melanoma, and acute myeloid leukemia, while inactivating mutations are associated with the genetic defect piebaldism.

References

1. Picardo, M., Cardinali, G. J Invest Dermatol. 2011 Jun; 131(6): 1182-5.
2. Yarden, Y., et al. EMBO J. 1987; 6(11): 3341-51.

Storage/Stability

Up to 6 months when stored as recommended.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

cKIT Assay Kit

PRODUCTS

SERVICES

RESOURCES

COMPANY