cKit, His-tag Recombinant

Catalog #

40250

$375 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human c-Kit also known as KIT, PBT, CD117, Piebald Trait, SCFR, and Mast/Stem Cell Growth Factor Receptor, GenBank Accession No. NM_000222, a.a. 544-976 with N-terminal His-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=53 kDa.

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Synonyms

KIT, cKIT, c-KIT, PBT, CD117, Piebald Trait, SCFR, Mast/Stem Cell Growth Factor Receptor

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

83%

Supplied As

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM Imidazole, 20% Glycerol and 3 mM DTT.

53 kDa

Amino Acids

544–976(end)

Specific Activity

15 pmole/min/μg

Genbank #

NM_000222

UniProt #

P10721

Tag(s)

N-terminal His-tag

References

1. Picardo M Cardinali G. J Invest Dermatol.2011 Jun;131(6):1182-5.
2. Yarden, Y., et al. EMBO J. 6 (11), 3341-3351 (1987)

Storage/Stability

At least 6 months at –80°C

Assay Conditions

40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 1 mM DTT using 0.2 mg/ml Poly-(Glu4:Tyr)- biotin substrate and 100 μM ATP. Reaction was done at 30°C for 30 min. ATP reduction was monitored using ADPGlo from Promega.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40250 Lot 110405.pdf

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