CDK12/Cyclin K, GST-Tags Recombinant

Catalog #

101235

$390 *

Size: 10 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Complex of CDK19 and Cyclin proteins. The two proteins are co-expressed and affinity purified.

Recombinant human CDK1 (cyclin dependent kinase 12), encompassing amino acids 658-1490(end). This construct contains an N-terminal GST-tag and includes a Thrombin Cleavage Site to facilitate removal of the GST portion. The recombinant protein was affinity purified.

Recombinant human Cyclin K (CCNK), full length, encompassing amino acids 2-580(end). This construct contains an N-terminal GST-tag and includes a Thrombin Cleavage Site to facilitate removal of the GST portion. The recombinant protein was affinity purified.

●

Synonyms

Cyclin-dependent kinase 12, Cdc2-related kinase, arginine/serine-rich, CrkRS, Cell division cycle 2-related protein kinase 7, CDC2-related protein kinase 7, Cell division protein kinase 12, hCDK12, CRK7, KIAA0904, Cyclin-K, CCNK, CPR4, CDK-12

●

Product Data Gallery

4-20% SDS-Page Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

CDK12 (GST-658-1490(end)) / CyclinK (GST-2-580(end))

Host Species/Expression System

Sf9

Purity

≥80%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT

CDK12: 119 kDa
Cyclin K: 90 kDa

Amino Acids

CDK12: 658-1490(end)
Cyclin K: 2-580(end)

Specific Activity

3 pmol/min/µg

Genbank #

CDK12: NM_016507
Cyclin K: NM_001099402

UniProt #

CDK12: Q9NYV4
Cyclin K: O75909

Tag(s)

N-terminal GST-tags

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was done according to CDK12/Cyclin K Kinase Assay Kit (BPS Bioscience #78298) with CDK12/Cyclin K titrated from 4000 ng/rxn to 0 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

101235 Lot 210927

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

CDK12/Cyclin K, GST-Tags Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY