CDK9/CyclinK, GST-Tag Recombinant
Complex of recombinant human CDK9 (Cyclin-dependent kinase 9), full length, amino acids 2-372(end), and recombinant human CyclinK, full length, amino acids 2-354(end). The CDK9 construct contains an N-terminal GST tag followed by a PreScission protease cleavage site, while the CyclinK construct contains an N-terminal GST-tag followed by a thrombin cleavage site. The proteins were co-expressed and purified as a complex.
≥90%
Aqueous buffer solution.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT
CDK9 (Cyclin-dependent kinase 9) is the catalytic subunit of the positive transcription elongation factor b (P-TEFb) complex, which phosphorylates the C-terminal domain of RNA polymerase II, a key player in the production of mature RNA. P-TEFb also includes CyclinT or CyclinK. CDK9 translocates to the nucleus following autophosphorylation, where it can associate with CyclinT or K. Hyperactivation of cyclins is known to result in cancer and the development of resistance to therapy, and CDK9-CyclinK is involved in genome integrity. Lack of CDK9 or CyclinK was shown to impair cell recovery after cells were stressed and potentiated DNA damage. Further understanding of the role of CKD9/CyclinK in health and disease and how to modulate it may prove beneficial for fields such as cancer therapy.
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