Chemi-Verse™ CDK9/CyclinK Kinase Assay Kit

Catalog #

82237

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Chemi-Verse™ CDK9/CyclinK Kinase Assay Kit is designed to measure CDK9 (cyclin dependent kinase 9)/Cyclin K kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant CDK9/CyclinK complex, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

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Synonyms

Cyclin-Dependent 9, CDK9: CDC2L4, C-2k, TAK, PITALRE CyclinK: CCNK, CPR4, MGC9113, CDK-9

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Product Data Gallery

Inhibition of CDK9/CyclinK kinase activity by Dinaciclib

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
102119	CDK9/CyclinK, GST-Tag*	20 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
	PDKtide (1 mg/ml)	500 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

CDK9: P50750; CyclinK: O75909

Background

Cyclin-dependent kinase 9 (CDK9) is the catalytic subunit of the positive transcription elongation factor b (P-TEFb) complex, which phosphorylates the C-terminal domain of RNA polymerase II, a key player in the production of mature RNA. P-TEFb also includes cyclinT or cyclinK. CDK9 translocates to the nucleus once it gets autophosphorylated, where it can associate with cyclinT or K. Hyperactivation of cyclins is known to result in cancer and the development of resistance to therapy, and CDK9-cyclinK is involved in genome integrity. Lack of CDK9 or cyclinK was shown to impair cell recovery after cells were stressed and potentiated DNA damage. Further understanding of the role of CKD9/cyclinK in health and disease and how to modulate it may prove beneficial for fields such as cancer therapy.

References

Napolitano G, et al., 2003 J Cell Physiol. 197(1):1-7.
Yu D. and Cortez D., 2011 Cell Cycle 10(1):28-32.
Li J., et al., 2022 J Med Chem. 65(16):11034-11057.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinases

Regulatory Status

Research Use Only

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Chemi-Verse™ CDK9/CyclinK Kinase Assay Kit

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