CDK13/Cyclin K, GST-Tags Recombinant

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Catalog #

101128

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Description

Complex of human CDK13 (cyclin dependent kinase 13) and human cyclin K. The proteins were co-expressed in Sf9 insect cells along with S. cerevisiae CAK1 (Serine/threonine-protein kinase CAK1).

Recombinant human CDK13, encompassing amino acids 516-1512 (end), contains an N-terminal GST-tag. Recombinant human Cyclin K, full length encompassing amino acids 1-580 (end), also contains an N-terminal GST-tag. Recombinant S. cerevisiae CAK1, encompassing amino acids 2-368 (end), contains a FLAG-tag. The proteins were purified using the GST tags.

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Synonyms

CHED, CDC2L, CDC2-related protein kinase 5, Cell division cycle 2-like protein kinase 5, CDC2L5, CHDFIDD, Cell division protein kinase 13, hCDK13, CCNK, CPR4, IDDHDF, CAK-1, CDK-activating kinase, CDK-13

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

Product Info

Storage and Usage

Citations

Species

Human

Construct

CDK13 (GST-516-1512(end)) / Cyclin K (GST-2-580(end))

Host Species/Expression System

Sf9; Coexpressed with CAK1, S. Cerevisiae (FLAG-2-368(end))

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT

CDK13: 138 kDa; Cyclin K: 90 kDa

Amino Acids

CDK13: 516-1512(end); Cyclin K: 2-580(end)

Genbank #

CDK13: NM_003718; Cyclin K: NM_001099402

UniProt #

CDK13: Q14004; Cyclin K: O75909

Tag(s)

N-terminal GST-tag

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Datasheets/Protocols

101128 Lot 210719G

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CDK13/Cyclin K, GST-Tags Recombinant

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