BTK, GST-His-Tag Recombinant

Catalog #

40405

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human BTK also known as Bruton Tyrosine Kinase, ATK, BPK and Tyrosine-Protein Kinase BTK, GenBank Accession No. NM_000061, a.a 1-659(end) with N-terminal GST-His tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=106 kDa.

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Synonyms

BTK, Bruton's tyrosine kinase BTK, ATK, BPK, Tyrosine-Protein Kinase BTK

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥85%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 10 mM glutathione, 50% glycerol, and 3 mM DTT.

106 kDa

Amino Acids

full length

Specific Activity

23 pmol/min/μg.

Genbank #

NM_000061

UniProt #

Q06187

Tag(s)

N-terminal GST-His-tag

References

1. Liljeroos M., et al., Cell Signal. 2007 Mar,19(3):625-33.
2. Jou ST., et al., Int J Immunogenet. 2006 Oct,33(5):361-369

Storage/Stability

At least 6 months at –80°C

Assay Conditions

40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 1 mM DTT using 0.2 mg/ml Poly-(Glu4:Tyr)-biotin substrate and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Cytoplasmic Tyr

Regulatory Status

Research Use Only

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Datasheets/Protocols

40405 Lot 181019-GB.pdf

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BTK, GST-His-Tag Recombinant

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