ITK Kinase Assay Kit

Catalog #

78429

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The ITK Kinase Assay Kit is designed to measure ITK kinase activity for screening and profiling applications using ADP-Glo^® as a detection reagent.

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Synonyms

Tyrosine-protein kinase ITK/TSK, Interleukin-2-inducible T-cell kinase, IL-2-inducible T-cell kinase, Kinase EMT, T-cell-specific kinase, Tyrosine-protein kinase Lyk, EMT, LYK

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The assay kit comes in a convenient 96-well format, with enough purified recombinant ITK kinase, kinase substrate, ATP and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

ADP-Glo® Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
40445	ITK*	5 µg	-80°C
78426	Kinase assay buffer 2 (5x)	1.5 ml	-20°C
79686	ATP (500 µM)	100 µl	-20°C
40217	Poly-(Glu4:Tyr) (10 mg/ml)	100 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

Q08881

Background

Tyrosine-protein kinase ITK/TSK, also known as Lyk or IL2-inducible T cell kinase, is a cytosolic tyrosine kinase expressed in T cells. ITK contains protein binding domains SH2 and SH3, which allows the kinase to be recruited by tyrosine-phosphorylated proteins, notably upon activation of the T Cell Receptor (TCR). It plays an important in TCR signal transduction pathways and ultimately leads to the activation of transcription factor NFAT (nuclear activator of activated T cells), cytokine secretion, and T cell differentiation and expansion.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only

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ITK Kinase Assay Kit

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