Chemi-Verse BTK Kinase Assay Kit

Catalog #

82298

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Chemi-Verse™ BTK Kinase Assay Kit is designed to measure BTK (Bruton’s tyrosine kinase) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified BTK, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

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Synonyms

Tyrosine-protein kinase BTK, Agammaglobulinemia tyrosine kinase (ATK), B-cell progenitor kinase (BPK), Bruton tyrosine kinase, AGMX1, ATK, BPK

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Product Data Gallery

Inhibition of BTK kinase activity by Ibrunitib.

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
40405	BTK, GST-His-Tag*	20 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
40217	Poly-(Glu, Tyr 4:1) (10 mg/ml)	50 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

Q06187

Background

Bruton’s tyrosine kinase or BTK, is a non-receptor kinase protein found in hematopoietic and plasma cells. It is involved in functionality, maturation, and apoptosis of B cells, in addition to regulating signals in the MAPK (mitogen-activated protein kinase), PI3K (phosphoinositide 3-kinase) and NF-κB (nuclear factor kappa B) pathways. The BTK pathway has implications for a number of autoimmune disorders including isolated growth hormone deficiency type III and rheumatoid arthritis. BTK inhibitors have been used in the treatment of inflammatory diseases and hematological disorders including cancer. For instance, Ibrutinib (also known as PCI-32765), is used in the clinic for the treatment of CLL (chronic lymphocytic leukemia), MCL (mantle cell lymphoma) and GvHD (graft versus host disease) and other diseases. However, low-selectivity BTK inhibitors have severe side effects, so further refinement is necessary. Development of highly selective BTK inhibitors will lead to greater benefits in the treatment of cancer and immune diseases.

References

Rawlings D., et al., 1993 Science 261(5519):358-361.
Alrouji M., et al., 2023 Scientific Reports 13: 17684.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinases

Regulatory Status

Research Use Only