3CL Protease (P252L) (SARS-CoV-2) Recombinant

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Catalog #

101692

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Description

Recombinant SARS-CoV-2 3C-like Protease, full length, encompassing amino acids 1-306(end). This construct does not contain a tag. The recombinant protein contains the mutation of interest (P252L) and is affinity purified.

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Synonyms

Mpro, Main protease, native 3CL, 3C-like protease, SARS protease, SARS-ncov protease, nsp5

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

3CL Protease (P252L) Activity

3CL Protease (P252L) (SARS-CoV-2) inhibition curves using a fluorogenic assay (#78839). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

SARS-CoV-2

Construct

3CL Protease (P252L) (1-306(end)) (SARS-CoV-2)

Mutation

P252L

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

34 kDa

Amino Acids

1-306(end)

Genbank #

YP_009725301

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay run according to 3CL Protease (P252L) (SARS-CoV-2) Assay Kit (BPS Bioscience #78839) with various amounts of 3CL Protease (P252L).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Coronavirus

Regulatory Status

Research Use Only

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3CL Protease (P252L) (SARS-CoV-2) Recombinant

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