3CL Protease, MBP-tag, His-tag (SARS-CoV-1) Recombinant

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Catalog #

100739

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Description

Severe acute respiratory Coronavirus 3C-like protease (SARS-CoV-1 3CL Protease), a.a. 1-306(full length), with an N-terminal MBP-tag, and C-terminal His-tag, expressed in an E. coli expression system, MW=78.5 kDa.

This product has been cited 2 times.

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Synonyms

3C-like protease, MPro, SARS protease, SARS-ncov protease, sars-cov-1, Mpro, Main protease, nsp5

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

3CL Protease SARS-CoV-1 Activity

3CL protease (SARS-CoV-1) inhibition curve using a fluorogenic assay (#78015). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations2

Species

SARS-CoV-1

Host Species/Expression System

E. coli

Purity

≥85%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, 8 mM maltose.

78.5 kDa

Amino Acids

1-306(full length)

Specific Activity

>25 pmol/min/μg

Tag(s)

N-terminal MBP-tag, C-terminal His-tag

References

1. Zhang, L., et al., Science. 2020 Apr; 368(6489): 409-412
2. Hsu, M.F. et al., JBC. 2005 Mar; 280:31257-31266.

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3CL Protease, MBP-tag, His-tag (SARS-CoV-1) Recombinant

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