3CL Protease (MERS-CoV) Inhibitor Screening Assay Kit

Catalog #

78278

$1,315 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The 3CL Protease (MERS-CoV) Inhibitor Screening Assay Kit is designed to measure 3CL Protease activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps. A protease inhibitor cocktail is included as a positive control.

Assay Principle

3CL Protease (MERS-CoV) Assay Kit uses a custom-synthesized peptide which has a fluorescence group quenched by a specific quencher. This peptide works as a substrate for 3CL Protease (MERS-CoV). After proteolytic cleavage, fluorescence product is formed and detected at λexcitation=485 nm and λemission=528 nm.

Need us to run inhibitor screens or profile your compounds against 3CL Protease (MERS-CoV)? Check out our Protease Screening Services or Coronavirus Services.

This product has been cited 2 times.

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Product Data Gallery

Product Info

Storage and Usage

Citations2

Assay Kit Format

Fluorogenic

Supplied As

The kit comes in a convenient 96-well format, with purified 3CL Protease (MERS-CoV), fluorogenic substrate, and 3CL MERS Protease assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Adjustable micropipettor and sterile tips
Fluorescent microplate reader
Rotating or rocker platform

Format

Catalog #	Name	Amount	Storage
100757	3CL Protease, MBP-Tag, His-tag (MERS-CoV)*	300 µg	-80°C
78021	3CL MERS-CoV substrate (1 mM)	20 µl	-80°C
78022-M	3CL MERS-CoV buffer	25 ml	-20°C
	Protease Inhibitor cocktail (10 mM in DMSO)	20 µl	-80°C
79685	Black 96-well plate	1	Room Temp

Background

Middle East Respiratory Syndrome coronavirus (MERS-CoV) poses a significant threat to public health worldwide due to its ability to cause serious human disease with high mortality rates. MERS-CoV has become a global threat due to continuous outbreaks in countries on the Arabian Peninsula and the potential for spread to other countries. MERS-CoV 3CL protease is essential for viral replication; consequently, it is an attractive target for small molecule therapeutics.

References

Kankanamalage A., et al., Eur. J. Med. Chem. 2018, 150: 334-346.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Instructions for Use

See assay protocol for detailed instructions.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide

Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the reaction should be ≤1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Coronavirus, COVID-19

Regulatory Status

Research Use Only

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3CL Protease (MERS-CoV) Inhibitor Screening Assay Kit

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