ZAP70, GST-tag Recombinant

Catalog #

40490

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human ZAP70, also known as ZAP-70, zeta-chain associated protein kinase, syk-related tyrosine kinase, SRK, or STD, GenBank Accession No. NM001079, full length with N-terminal GST tag, MW = 96 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

ZAP-70, Zeta-Chain Associated Protein Kinase, Syk-Related Tyrosine Kinase, SRK, STD

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCL, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol and 1.6 mM glutathione

96 kDa

Amino Acids

full length

Specific Activity

20 pmole/min/μg

Genbank #

NM_001079

UniProt #

P43403

Tag(s)

N-terminal GST-tag

References

1. Ishaq, M., et al. J Immunol. 2007 Sep 15;179(6):3851-8.
2. Siggs, O., et al. Immunity. 2007 Dec;27(6):912-26.

Storage/Stability

>6 months at –80°C.

Assay Conditions

Assay was done in Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr) substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30oC for 45 min. Amount of ATP transferred was calculated using Kinase- Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Scientific Category

Kinase/Cytoplasmic Tyr

Regulatory Status

Research Use Only

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Datasheets/Protocols

40490_Lot140813-1.pdf

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