ZAP70 Kinase Assay Kit
The ZAP70 Kinase Assay Kit is designed to measure ZAP70 kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
- Kinase-Glo MAX (Promega #V6071)
- DTT (Dithiothreitol), 1M, optional
- Microplate reader capable of reading luminescence
- Adjustable micropipettor and sterile tips
- 30°C incubator
| Catalog # | Name | Amount | Storage |
| 40490 | ZAP70* | 5 µg | -80°C |
| 79334 | Kinase assay buffer 1 (5x) | 1.5 ml | -20°C |
| 79686 | ATP (500 µM) | 100 µl | -20°C |
| 40217 | Poly-(Glu4:Tyr) (10 mg/ml) | 100 µl | -20°C |
| 79696 | White 96-well plate | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube.
ZAP70 (Zeta-chain-associated protein kinase-70) is a cytosolic tyrosine kinase expressed in immune cells such as T cells, NK cells, and a subset of B cells. It is recruited by the T Cell Receptor (TCR) upon activation by antigen binding to regulate gene expression and immune cell proliferation. Overexpression of ZAP70 has been observed in B cell malignancies, while patients with defects in ZAP70 have no functional T cells. Malfunctioning of ZAP70 appears to be involved in the onset of autoimmune diseases. ZAP70 is a close homolog to tyrosine kinase SYK, also involved in immune responses.
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