Tie2, GST-tag Recombinant

Catalog #

40370

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human TEK tyrosine kinase, endothelial, also known as Tie2, (GenBank Accession No. NM_000459) a.a. 771-1124(end) with N-terminal GST-tag, MW= 66 kDa, expressed in baculovirus infected Sf9 cell expression system.

This product has been cited 1 time.

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Synonyms

TIE, TEK, Tyrosine Kinase With Immunoglobulin-Like And EGF-Like Domains 1

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Product Data Gallery

4-20 % SDS-PAGE Coomassie Staining

Tie2 Activity

Product Info

Storage and Usage

Citations1

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥80%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 3 mM DTT, and 20% glycerol

66 kDa

Amino Acids

771–1124(end)

Specific Activity

1400 pmol/min/μg.

Genbank #

NM_000459

UniProt #

Q02763

Tag(s)

N-terminal GST-tag

References

1. Murray, B.W., et al., Biochemistry. 2001 Aug 28,40(34):10243-53.
2. Yuan, H.T., et al., Mol Cell Biol. 2009 Apr,29(8):2011-22.

Storage/Stability

>6 months at –80°C.

Assay Conditions

Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, 2 μM substrate (Tyr Peptide 5, Z-lyte kinase assay kit from Invitrogen), and enzyme at 37°C for 1 hour.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40370_Lot71128.pdf

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