SUV39H1 Chemiluminescent Assay Kit

Catalog #

52006L

$750 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The SUV39H1 Chemiluminescent Assay Kit is designed to measure SUV39H1 activity for screening and profiling applications. The key to the SUV39H1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the ELISA ECL substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

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Synonyms

KMT1A, SUV39H1, Suppressor Of Variegation 3-9 Homolog 1 (Drosophila), Su(Var)3-9 Homolog 1

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

The SUV39H1 Chemiluminescent Assay Kit comes in a convenient format, with a 96-well strip plate precoated with histone H3 peptide substrate, primary antibody against methylated lysine residue of Histone H3, secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified SUV39H1 enzyme for 96 enzyme reactions.

Format

Catalog #	Component	Amount	Storage
51070	SUV39H1 human enzyme*	10 µg	-80°C	Avoid freeze/thaw cycles!
52120	100 µM S-adenosylmethionine	250 µl	-80°C
52140A	Primary antibody 1	100 µl	-80°C
52130H	Secondary HRP-labeled antibody 1	10 µl	-80°C
52160	4x HMT assay buffer 1	3 ml	-20°C
79556	Blocking buffer 1	50 ml	+4°C
79670	ELISA ECL substrate A (transparent bottle)	6 ml	Room Temp.
79670	ELISA ECL substrate B (brown bottle)	6 ml	Room Temp.
	96-well strip plate precoated with histone substrate	1 plate	+4°C

*The concentration of SUV39H1 is lot-specific and will be indicated on the tube containing the enzyme.

UniProt #

O43463

References

Dillon S.C., et al. 2005. Genome Biology 6:227.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only