SUV39H1, GST-tag Recombinant

Catalog #

51070

$455 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human SUV39H1, GenBank Accession No. NM_003173, a.a. 82-412, with N-terminal GST-tag, MW= 64 kDa, expressed in an E. coli expression system.

This product has been cited 2 times.

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Synonyms

KMT1A

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Product Data Gallery

Specific Activity

10% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations2

Species

Human

Host Species/Expression System

E. coli

Purity

≥70%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT.

64 kDa

Amino Acids

82–412

Specific Activity

≥0.1 pmol/min/µg

Genbank #

NM_003173

UniProt #

O43463

Tag(s)

N-terminal GST-tag

References

1. Kang, M.Y. et al., Int. J. Cancer 121, 10, 2192-2197, 2007.
2. Spensberger, D. and Delwel, R. FEBS Lett. 582, 18, 2761-2767, 2008.

Storage/Stability

>6 months at –80°C.

Assay Conditions

50 µl reaction mix (50 mM Tris pH 8.8, 5 mM MgCl₂, 4 mM DTT, 0.05% Tween-20, 40 µM S-adenosylmethionine, and 10-100 ng SUV39H1) is added to the wells of a substrate-coated Neutravidin black plate (G-Biosciences). Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only

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Datasheets/Protocols

51070_Lot140817-G1.pdf
51070_Lot140114-G.pdf

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SUV39H1, GST-tag Recombinant

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