RIPK2, GST-tag Recombinant

Catalog #

40173

$390 *

Size: 10 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Recombinant human RIPK2 (receptor interacting serine/threonine kinase 2), encompassing amino acids 2-299. This construct contains an N-terminal GST tag followed by a PreScission Protease Cleavage Site for the removal of the GST-tag. The recombinant protein was affinity purified.

●

Synonyms

RICK; RIP2; CARD3; CARDIAK; CCK; GIG30

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

RIPK2 (GST-Pre-2-299)

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 500 mM NaCl, 2.5 mM KCl, 0.02% Tween-20, 3 mM DTT and 10% glycerol

61 kDa

Amino Acids

2-299

Specific Activity

≥15 pmol/min/μg

Genbank #

NM_003821

UniProt #

O43353

Tag(s)

N-terminal GST-tag

Background

RIPK2 (RIP2; RICK) is a death domain-containing protein kinase encoding a predicted 540-amino acid protein which contains an N-terminal serine/threonine kinase catalytic domain and a C-terminal caspase activation and recruitment domain. RIPK2 is thought to regulate apoptosis induced by the FAS receptor pathway (1). RIPK2 has been shown to specifically interact with the CARD of ICE/caspase-1 and this interaction correlates with the processing of pro-caspase-1 and the formation of the active caspase-1 p20 (2).

References

1. Inohara, N. et al: RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis. J. Biol. Chem. 273: 12296-12300, 1998. Note: Erratum: J. Biol. Chem 273: 18675 only, 1998.
2. Thome, M. et al: Identification of CARDIAK, a RIP-like kinase that associates with caspase-1. Curr. Biol. 8: 885-888, 1998.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was done in a Kinase buffer (BPS Bioscience #79334) using MBP (0.2 mg/ml) as a substrate with 20 μM ATP at 30°C for 45 minutes. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

40173 Lot 190118-G2

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

RIPK2, GST-tag Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY