RIPK1, GST-Th-Tag Recombinant

Catalog #

40371

$420 *

Size: 10 µg

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Description

Recombinant human RIPK1 (receptor-interacting serine-threonine kinase 1), encompassing amino acids 1-327 containing the protein kinase domain. This construct contains an N-terminal GST-tag. A short peptide placed between GST and the protein includes a Thrombin Cleavage Site to facilitate removal of the GST portion. The recombinant protein was affinity purified.

This product has been cited 1 time.

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Synonyms

Receptor-interacting serine/threonine-protein kinase, Cell death protein RIP, Receptor-interacting protein 1, RIP-1, RIPK1, RIP, RIP1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

RIPK1 Activity

Product Info

Storage and Usage

Citations1

Species

Human

Construct

RIPK1 (GST-Th-1-327)

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% Glycerol.

64 kDa

Amino Acids

1-327

Genbank #

NM_003804

UniProt #

Q13546

Tag(s)

N-terminal GST-Th-Tag

Background

RIPK1 or Receptor Interacting Protein Kinase 1 is a serine/threonine kinase that was originally identified as interacting with the cytoplasmic domain of FAS. RIPK1 has been deemed as an important element in the signal transduction machinery that mediates programmed cell death. RIPK1 has been shown to interact with TRADD, TRAF1 TRAF2 and TRAF3 and TRADD can act as an adaptor protein to recruit RIPK1 to the TNFR1 complex in a TNF-dependent process. TNFα is capable of activating the noncanonical NF-κB pathway, but this activation of this pathway is negatively regulated by RIPK1.

References

1. Hsu, H. et al., Immunity 4: 387-396, 1996.
2. Kim, J Y. et al., J Cell Sci. 2011 Feb 15;124(Pt 4):647-56.

Application References:

1. The Bcr-Abl inhibitor GNF-7 inhibits necroptosis and ameliorates acute kidney injury by targeting RIPK1 and RIPK3 kinases

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was done according to RIPK1 Kinase Assay Kit (BPS Bioscience #79560) with RIPK1 (BPS Bioscience #40371) titrated at various concentrations.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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RIPK1, GST-Th-Tag Recombinant

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