The RIPK1 Kinase Assay Kit is designed to measure RIPK1 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent. MBP (Myelin Basic Protein) is a non-specific protein substrate that is used as a "universal substrate" for many in-vitro kinase activity assays. This protein is targeted by many serine/threonine kinases at conserved amino acids. We use the dephosphorylated version of the MBP substrate in our assays to determine the kinase-mediated phosphorylation of MBP. Our assays are not suitable for studying autophosphorylation of the kinase due to the presence of the MBP substrate.
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Synonyms
RCK, MAP3K19, receptor-interacting serine/threonine-protein kinase 1 isoform 1, receptor-interacting serine/threonine-protein kinase 1, serine/threonine-protein kinase RIP, cell death protein RIP, receptor-interacting protein 1, receptor (TNFRSF)-interact
The RIPK1 Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant RIPK1 enzyme, RIPK1 substrate, ATP and kinase assay buffer for 100 enzyme reactions.
RIPK1 is a member of the receptor-interacting protein kinase (RIP) family. It plays an important role in cell survival as well as cell death including apoptosis and necroptosis. RIPK1 is highly expressed in Alzheimer’s disease (AD) brains. Importantly, it was shown that inhibition of RIPK1 results in decrease of amyloid and inflammation levels as well as accelerating amyloid beta peptide (Aβ) degradation by microglia in vitro.
References
Ofengeim D., Proc. Natl. Acad. Sci. USA 114(41): E8788-E8797 (2017)
Storage/Stability
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Instructions for Use
See assay kit data sheet for detailed protocol.
Shipping Temperature
-80°C
Warnings
Avoid freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.
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