PRKG1, GST-tag Recombinant

Catalog #

40175

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human PRKG1, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.

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Synonyms

PGK, CGKI, PRKG1B, PRKGR1B, FLJ36117, MGC71944, cGKI-BETA, cGKI-alpha, DKFZp686K042

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥75%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

100 kDa

Amino Acids

full length

Genbank #

NM_006258

UniProt #

Q13976

Tag(s)

N-terminal GST-tag

Background

PRKG1 is a homodimer, with each monomer containing a regulatory cGMP-binding domain and a catalytic domain (1). By Northern blot analysis PRKG1 was shown to be expressed at highest levels in bladder, uterus, adrenal gland, and fallopian tube. PRKG1 plays an important stimulatory role in platelet activation (2). Expression of recombinant PRKG1 in a reconstituted cell model enhanced von Willebrand factor-induced activation of the platelet integrin alpha-IIb/beta-3. Prkg1 knockout mice showed impaired platelet responses to VWF or low doses of thrombin and prolonged bleeding time. Human platelet aggregation induced by VWF or low-dose thrombin was inhibited by PRKG1 inhibitors but enhanced by cGMP.

References

1. Orstavik, S. et al: Characterization of the human gene encoding the type I-alpha and type I-beta cGMP-dependent protein kinase (PRKG1). Genomics 42: 311-318, 1997.
2. Li, Z. et al: A stimulatory role for cGMP-dependent protein kinase in platelet activation. Cell 112: 77-86, 2003.

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PRKG1, GST-tag Recombinant

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