PRKG2, GST-tag Recombinant

Catalog #

40170

$410 *

Size: 10 µg

Qty

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Description

Recombinant human PRKG2, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.

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Synonyms

cGKII, PRKGR2

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥95%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

112 kDa

Amino Acids

full length

Genbank #

NM_006259

UniProt #

Q13237

Tag(s)

N-terminal GST-tag

Background

PRKG2 is a member of the cGMP-dependent protein kinase family and is highly expressed in brain, lung, and intestinal mucosa. PRKG2 mediates intestinal secretion of water and electrolytes induced by the E. coli toxin STa and the intestinal peptide guanylin (1). Mice deficient in PRKG2 are resistant to E. coli STa and developed dwarfism which is caused by a severe defect in endochondral ossification at the growth plates. PRKG2 phosphorylates SOX9 and attenuates SOX9 function by inhibiting its nuclear entry. PRKG2 is a molecular switch that couples the cessation of proliferation and the start of hypertrophic chondrocyte differentiation through attenuating SOX9 function (2).

References

1. Pfeifer, A. et al: Intestinal secretory defects and dwarfism in mice lacking cGMP-dependent protein kinase II. Science 274: 2082-2084, 1996.
2. Chikuda, H. Et al: Cyclic GMP-dependent protein kinase II is a molecular switch from proliferation to hypertrophic differentiation of chondrocytes. Genes Dev. 18: 2418-2429, 2004.

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PRKG2, GST-tag Recombinant

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